Microbial genomics and antimicrobial susceptibility testing.
نویسندگان
چکیده
INTRODUCTION Antimicrobial susceptibility testing is key in modern clinical microbiology. With pandemic emergence of (multi-)antibiotic resistance, methods to detect and quantify resistance of clinically important bacterial species are imperative. Historically, antimicrobial susceptibility testing (AST) was mostly performed using methods relying on bacterial growth. Such methods may be time-consuming and more rapid alternatives have been actively sought for. Areas covered: Among the new AST methods there are many that focus on detection of causal resistance genes and/or gene mutations. The approaches most used are based on nucleic acid amplification and, more recently, high-throughput (next generation) sequencing of amplified targets and complete microbial genomes. The authors provide a review of PCR-mediated and genomic AST methods used for human and veterinary pathogens and show where these approaches work well or may become difficult to interpret. Expert commentary: Microbial genome sequencing will play an important role in the field of AST, but there remain issues to be resolved. These include the development of user friendly data analysis, reducing the duration and cost of sequencing and comprehensiveness of the databases. In addition, clinical evaluation studies need to be performed involving real-life patients.
منابع مشابه
Antimicrobial susceptibility testing of Mannheimia haemolytica and Pasteurella multocida isolated from calves with dairy calf pneumonia
This study evaluated the nasopharyngeal microbial flora and antimicrobial susceptibility patterns of the one hundred and thirty Holstein calves with dairy calf pneumonia from dairy farms of Mashhad Suburb between September 2002 and August 2003 . The most common micro-organisms isolated were Pasteurella multocida 80 (61.54%), Mannheimia haemolytica 41 (31.54%), Bacillus sp. 15 (11.54%), Staphy...
متن کاملMicrobial Etiology and Antimicrobial Susceptibility of Bactria Implicated in Urinary Tract Infection in Tehran, Iran
Background and Objectives: Urinary tract infections (UTI) are one of the most common infectious diseases with different microbial agent and antimicrobial resistant pattern in hospitalized patients and outpatients. In order to assess the adequacy of therapy, knowledge of prevalence and resistance pattern of the bacteria is necessary. The main aim of this study was to evaluate the prevalence and ...
متن کاملComparison of Disc Diffusion and E test methods for Antimicrobial Susceptibility Testing Of Vancomycin in Coagulase Negative Staphylococcus Isolated from Blood Culture
Background and Objective: Reliable methods of detection of antimicrobial resistance are of paramount importance in the treatment and management of infections caused by coagulase negative staphylococci (CoNS). The objective of the present study was to compare and evaluate the performance of disc diffusion and E test (Epsilometer test) methods for antimicrobial testing of coagulase ne...
متن کاملRapid method of microbial susceptibility testing.
A rapid method of microbial susceptibility testing is described. Bacterial growth is measured by determining the incorporation of (14)C-tryptophan into cellular protein in the presence of antimicrobial drugs.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Expert review of molecular diagnostics
دوره 17 3 شماره
صفحات -
تاریخ انتشار 2017